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  • Featured Product
  • KD/KO Validated

GDPD1 Polyclonal antibody

GDPD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27861-1-AP

Synonyms

GDE4, GDPD1, UGPQ

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDPD1 Polyclonal antibody (27861-1-AP)

    WB analysis using 27861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27861-1-AP (GDPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using GDPD1 Polyclonal antibody (27861-1-AP)

    IHC staining of human testis using 27861-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using GDPD1 Polyclonal antibody (27861-1-AP)

    IHC staining of human testis using 27861-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue, mouse ovary tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27861-1-AP targets GDPD1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDPD1 fusion protein Ag27371 相同性解析による交差性が予測される生物種
Full Name glycerophosphodiester phosphodiesterase domain containing 1
Calculated molecular weight 36 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC034432
Gene symbol GDPD1
Gene ID (NCBI) 284161
RRIDAB_2880996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GDPD1 antibody 27861-1-APDownload protocol
IHC protocol for GDPD1 antibody 27861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Differ

A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.

Authors - Xiaoyu Qi
View Article
humanWB

J Lipid Res

Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7

Authors - Keisuke Kitakaze
  • KO Validated
View Article