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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using GEFT Polyclonal antibody (17028-1-AP)

    WB analysis of human brain using 17028-1-AP (same clone as 17028-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 17028-1-AP (GEFT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17028-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GEFT Polyclonal antibody (17028-1-AP)

    IP experiment of HEK-293 using 17028-1-AP (same clone as 17028-1-PBS)

    IP result of anti-GEFT (IP:17028-1-AP, 4ug; Detection:17028-1-AP 1:400) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 17028-1-PBS in a different storage buffer formulation.

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GEFT Polyclonal antibody, PBS Only

GEFT Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 17028-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17028-1-PBS targets GEFT in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEFT fusion protein Ag10436 相同性解析による交差性が予測される生物種
Full Name RhoA/RAC/CDC42 exchange factor
Calculated molecular weight64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012860
Gene Symbol GEFT
Gene ID (NCBI) 115557
RRIDAB_2279083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VW2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).