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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEFT Monoclonal antibody, PBS Only

GEFT Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

rabbit, pig, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

5A11F8

Cat no : 66012-1-PBS

Synonyms

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse brain using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat brain using 66012-1-Ig (same clone as 66012-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GEFT Monoclonal antibody (66012-1-Ig)

    FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66012-1-PBS targets GEFT in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, pig, rat, mouse, human samples.

Tested Reactivity rabbit, pig, rat, mouse, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GEFT fusion protein Ag10819 相同性解析による交差性が予測される生物種
Full Name RhoA/RAC/CDC42 exchange factor
Calculated molecular weight64 kDa
Observed molecular weight 53 kDa, 63 kDa
GenBank accession numberBC012860
Gene symbol GEFT
Gene ID (NCBI) 115557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).