• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse brain using 66012-1-Ig (same clone as 66012-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat brain using 66012-1-Ig (same clone as 66012-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GEFT Monoclonal antibody (66012-1-Ig)

    IF Staining of HepG2 using 66012-1-Ig (same clone as 66012-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEFT antibody (66012-1-Ig, Clone: 5A11F8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GEFT Monoclonal antibody (66012-1-Ig)

    FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GEFT Monoclonal antibody, PBS Only

GEFT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66012-1-PBS
Clone No.5A11F8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66012-1-PBS targets GEFT in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GEFT fusion protein Ag10819 相同性解析による交差性が予測される生物種
Full Name RhoA/RAC/CDC42 exchange factor
Calculated molecular weight64 kDa
Observed molecular weight 53 kDa, 63 kDa
GenBank accession numberBC012860
Gene Symbol GEFT
Gene ID (NCBI) 115557
RRIDAB_11044192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86VW2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).