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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GEMIN4 Polyclonal antibody

GEMIN4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12408-1-AP

Synonyms

GEMIN4, HC56, HCAP1, HHRF 1, p97

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GEMIN4 Polyclonal antibody (12408-1-AP)

    WB analysis of HEK-293 using 12408-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GEMIN4 Polyclonal antibody (12408-1-AP)

    WB analysis of HeLa using 12408-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12408-1-AP (GEMIN4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GEMIN4 Polyclonal antibody (12408-1-AP)

    IP experiment of HEK-293 cells using 12408-1-AP

    IP result of anti-GEMIN4 (12408-1-AP for IP and Detection) with 293 cell lysate.

  • Immunoprecipitation (IP) experiment of HeLa cells using GEMIN4 Polyclonal antibody (12408-1-AP)

    IP experiment of HeLa using 12408-1-AP

    IP result of anti-GEMIN4 (IP:12408-1-AP, 5ug; Detection:12408-1-AP 1:200) with HeLa cells lysate 3100ug.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12408-1-AP targets GEMIN4 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GEMIN4 fusion protein Ag3099 相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 4
Calculated molecular weight 1058 aa, 120 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC020062
Gene symbol GEMIN4
Gene ID (NCBI) 50628
RRIDAB_10640891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GEMIN4, also named as P97, is a sub-unit of SMN complex. The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs). It plays an important role in the splicing of cellular pre-mRNAs. The express amount of GEMIN4 is about 8.79 ppm (http://pax-db.org/#!protein/984742). This antibody detects 97-110 kDa GEMIN4.

Protocols

Product Specific Protocols
WB protocol for GEMIN4 antibody 12408-1-APDownload protocol
IP protocol for GEMIN4 antibody 12408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols