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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GEMIN5 Recombinant antibody (84171-5-RR)

    WB analysis using 84171-5-RR (same clone as 84171-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GEMIN5 Recombinant antibody (84171-5-RR)

    IF Staining of HeLa using 84171-5-RR (same clone as 84171-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GEMIN5 Recombinant antibody (84171-5-RR)

    FC experiment of HEK-293 using 84171-5-RR (same clone as 84171-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84171-5-RR

    Affinity and Kinetic Characterization of 84171-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

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GEMIN5 Recombinant antibody, PBS Only

GEMIN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84171-5-PBS
Clone No.241205G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Gem-associated protein 5, Gem associated protein 5, 241205G7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84171-5-PBS targets GEMIN5 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GEMIN5 fusion protein Ag19220 相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 5
Calculated molecular weight 1508 aa, 169 kDa
Observed molecular weight 168-180 kDa
GenBank accession numberBC113614
Gene Symbol GEMIN5
Gene ID (NCBI) 25929
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TEQ6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.