Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis using 23935-1-AP (same clone as 23935-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 23935-1-AP (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
WB analysis of U-251 using 23935-1-AP (same clone as 23935-1-PBS)
WB result of GFAP antibody (23935-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)
IP result of anti-GFAP (IP:23935-1-AP, 4ug; Detection:23935-1-AP 1:20000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 23935-1-AP (same clone as 23935-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas slide using 23935-1-AP (GFAP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IF Staining of rat brain using 23935-1-AP (same clone as 23935-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 23935-1-AP (same clone as 23935-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23935-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GFAP Polyclonal antibody, PBS Only

GFAP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Cat No. 23935-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

glial fibrillary acidic protein

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23935-1-PBS targets GFAP in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GFAP fusion protein Ag20853 相同性解析による交差性が予測される生物種
Full Name	glial fibrillary acidic protein
Calculated molecular weight	432 aa, 50 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC013596
Gene Symbol	GFAP
Gene ID (NCBI)	2670
RRID	AB_2879367
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14136
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Target

Host

Species Reactivity

Species Reactivity

Conjugate