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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GFAP Monoclonal antibody, PBS Only

GFAP Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B2E10

Cat no : 60190-1-PBS

Synonyms

FLJ45472, GFAP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GFAP Monoclonal antibody (60190-1-Ig)

    WB analysis using 60190-1-Ig (same clone as 60190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    WB analysis of pig brain using 60190-1-Ig (same clone as 60190-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    WB analysis of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using GFAP Monoclonal antibody (60190-1-Ig)

    WB analysis of U-251 using 60190-1-Ig (same clone as 60190-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IP experiment of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

    IP result of anti-GFAP (IP:60190-1-Ig, 5ug; Detection:60190-1-Ig 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of human brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of human gliomas using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of human gliomas using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IHC staining of rat brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IF Staining of rat brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (60190-1-Ig, Clone: 4B2E10 ) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GFAP Monoclonal antibody (60190-1-Ig)

    IF Staining of mouse brain using 60190-1-Ig (same clone as 60190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GFAP antibody (60190-1-Ig, Clone: 4B2E10 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60190-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60190-1-PBS targets GFAP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFAP fusion protein Ag10452 相同性解析による交差性が予測される生物種
Full Name glial fibrillary acidic protein
Calculated molecular weight 432 aa, 50 kDa
Observed molecular weight 45-52 kDa
GenBank accession numberBC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Function
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.
Tissue specificity
GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).
Involvement in disease
  • Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
  • Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.
Isoforms
Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.
Post-translational modifications
Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).
Cellular localization
GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.