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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GFAP Recombinant antibody, PBS Only

GFAP Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat , pig and More (1)

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C6

Cat no : 81063-1-PBS

Synonyms

FLJ45472, GFAP

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using GFAP Recombinant antibody (81063-1-RR)

    WB analysis of U-251 using 81063-1-RR (same clone as 81063-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GFAP Recombinant antibody (81063-1-RR)

    WB analysis using 81063-1-RR (same clone as 81063-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IHC staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of rat brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GFAP Recombinant antibody (81063-1-RR)

    IF Staining of mouse brain using 81063-1-RR (same clone as 81063-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81063-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81063-1-PBS

    Affinity and Kinetic Characterization of 81063-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81077-1-RR against Human GFAP were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Published Applications

IHCSee 1 publications below

Product Information

81063-1-PBS targets GFAP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GFAP fusion protein Ag10423 相同性解析による交差性が予測される生物種
Full Name glial fibrillary acidic protein
Calculated molecular weight 432 aa, 50 kDa
GenBank accession numberBC013596
Gene symbol GFAP
Gene ID (NCBI) 2670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis

Authors - Xiaoxiao Guo
View Article