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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GFI1 Polyclonal antibody

GFI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14198-1-AP

Synonyms

GFI 1, GFI1, Zinc finger protein 163, Zinc finger protein Gfi 1, ZNF163

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of K-562 using 14198-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of mouse thymus using 14198-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of mouse thymus using 14198-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of PC-3 using 14198-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of HL-60 using 14198-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of K-562 using 14198-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of RAW264.7 using 14198-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of Raji using 14198-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using GFI1 Polyclonal antibody (14198-1-AP)

    WB analysis of HL-60 using 14198-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14198-1-AP (GFI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, mouse thymus tissue, PC-3 cells, Raji cells, RAW264.7

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14198-1-AP targets GFI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFI1 fusion protein Ag5401 相同性解析による交差性が予測される生物種
Full Name growth factor independent 1 transcription repressor
Calculated molecular weight 45 kDa
Observed molecular weight 62-66 kDa
GenBank accession numberBC032751
Gene symbol GFI1
Gene ID (NCBI) 2672
RRIDAB_2109976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GFI is a transcriptional repressor that is transiently induced during T-cell differentiation. It has critical roles in enhancing T helper-2 (Th2) cell expansion and in repressing induction of Th17 and CD103-positive inducible regulatory T (Treg) cells [PMID: 19188499]. GFI1 transcriptional regulator oncoprotein plays a crucial role in hematopoietic, inner ear, and pulmonary neuroendocrine cell development and governs cell processes as diverse as self-renewal of hematopoietic stem cells, proliferation, apoptosis, differentiation, cell fate specification, and oncogenesis (PMID: 18805794). The calculated molecular weight of GFI1 protein is about 45 kDa, but the modification of ubiquitinated GIF1 protein is about 62-66 kDa (PMID: 12721361).

Protocols

Product Specific Protocols
WB protocol for GFI1 antibody 14198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article