GFM1 Polyclonal antibody

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14274-1-AP

Synonyms

EGF1, EF-Gmt, EFGM, EFG1, EF Gmt


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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, human heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14274-1-AP targets GFM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFM1 fusion protein Ag5616 相同性解析による交差性が予測される生物種
Full Name G elongation factor, mitochondrial 1
Calculated molecular weight 86 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC049210
Gene symbol GFM1
Gene ID (NCBI) 85476
RRIDAB_2110140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

Protocols

Product Specific Protocols
WB protocol for GFM1 antibody 14274-1-APDownload protocol
IHC protocol for GFM1 antibody 14274-1-APDownload protocol
IF protocol for GFM1 antibody 14274-1-APDownload protocol
IP protocol for GFM1 antibody 14274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.

Authors - Sarah E Calvo
mouseWB,CoIP

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
human,mouseWB

Cells

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Authors - Jana Key
humanWB

Mitochondrion

Toward genotype phenotype correlations in GFM1 mutations.

Authors - Galmiche Louise L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Miguel (Verified Customer) (03-21-2022)

WB analysis of mouse liver using 14274-1-AP. Mouse liver homogenates in Ripa buffer were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:1000 incubated at 4°C O/N. KI/KO: Compound heterozygous knock-in/knock-out (Gfm1R671C/-) mouse model (1). WT: Wild type control mice (Gfm1+/+). (1) ) Molina-Berenguer M, Vila-Julià F, Pérez-Ramos S, Salcedo-Allende MT, Cámara Y, Torres-Torronteras J, Martí R. Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations. FASEB J. 2022 Jan;36(1):e22091. doi: 10.1096/fj.202100819RRR. PMID: 34919756.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver homogenate
GFM1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver homogenate (Cat no:14274-1-AP)