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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aequorea victoria GFP tag Monoclonal antibody, PBS Only

GFP tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E10H7

Cat no : 66002-1-PBS

Synonyms

CFP, eGFP, eYFP, GFP, YFP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GFP tag Monoclonal antibody (66002-1-Ig)

    WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

    HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of recombinant protein using GFP tag Monoclonal antibody (66002-1-Ig)

    WB analysis of recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

    WB result of eGFP fusion protein with anti-eGFP tag (66002-1-Ig) with GFP fusion protein at varous dilutions. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using GFP tag Monoclonal antibody (66002-1-Ig)

    WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using GFP tag Monoclonal antibody (66002-1-Ig)

    WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using GFP tag Monoclonal antibody (66002-1-Ig)

    IP experiment of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:2000) with the vector plasmid pEGFP-N1 Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using GFP tag Monoclonal antibody (66002-1-Ig)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig (GFP tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using GFP tag Monoclonal antibody (66002-1-Ig)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66002-1-PBS targets GFP tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberU73901
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.