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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Aequorea victoria GFP tag Recombinant antibody, PBS Only

GFP tag Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241614B3

Cat no : 81835-2-PBS

Synonyms

241614B3

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using GFP tag Recombinant antibody (81835-2-RR)

    WB analysis of Recombinant protein using 81835-2-RR (same clone as 81835-2-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using GFP tag Recombinant antibody (81835-2-RR)

    WB analysis of Transfected HEK-293 using 81835-2-RR (same clone as 81835-2-PBS)

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected COS-7 using GFP tag Recombinant antibody (81835-2-RR)

    IF Staining of Transfected COS-7 using 81835-2-RR (same clone as 81835-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected COS-7 cells using GFP tag antibody (81835-2-RR, Clone: 241614B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81835-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81835-2-RR

    Affinity and Kinetic Characterization of 81835-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81835-2-RR against Aequorea victoria GFP tag were performed. The affinity constant is 0.619 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81835-2-PBS targets GFP tag in WB, IF/ICC, Indirect ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Reactivity aequorea victoria, recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberM62653
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.