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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GFRA2 Polyclonal antibody, PBS Only

GFRA2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55038-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    WB analysis of human placenta using 55038-1-AP (same clone as 55038-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55038-1-AP (GFRA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    IHC staining of human lung cancer using 55038-1-AP (same clone as 55038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55038-1-AP (GFRA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GFRA2 Polyclonal antibody (55038-1-AP)

    IHC staining of human lung cancer using 55038-1-AP (same clone as 55038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55038-1-AP (GFRA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55038-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55038-1-PBS targets GFRA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GDNF family receptor alpha 2
Calculated molecular weight 52 kDa
Observed molecular weight 41-45 kDa
GenBank accession numberNM_001495
Gene symbol GFRA2
Gene ID (NCBI) 2675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-52 kDa. This antibody is specific to GFRA2.