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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GFRAL Polyclonal antibody (28335-1-AP)

    WB analysis using 28335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28335-1-AP (GFRAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GFRAL Polyclonal antibody

GFRAL Polyclonal Antibody for WB, ELISA
Cat No. 28335-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

UNQ9356, GRAL, C6orf144, bA360D14.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28335-1-AP targets GFRAL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFRAL fusion protein Ag28504 相同性解析による交差性が予測される生物種
Full Name GDNF family receptor alpha like
Calculated molecular weight 45 aa, 85 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberNM_207410
Gene Symbol GFRAL
Gene ID (NCBI) 389400
RRIDAB_3669653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GFRAL (GDNF family receptor alpha-like), also called C6orf144, is the brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses (PMID: 28846097, 28846098, 28846099, 28953886, 36630958). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PMID: 32661391). GFRAL is a single-pass membrane protein, and localizes to the plasma membrane. It is mainly expressed in the hindbrain, and its mainstream molecular weight is 45-60 kd.

Protocols

Product Specific Protocols
WB protocol for GFRAL antibody 28335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols