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  • Featured Product
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GGA2 Polyclonal antibody

GGA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10356-1-AP

Synonyms

VHS domain and ear domain of gamma-adaptin, Vear, Golgi-localized, gamma ear-containing, ARF-binding protein 2, Gamma-adaptin-related protein 2, ADP-ribosylation factor-binding protein GGA2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of HeLa using 10356-1-AP

    WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse brain tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse brain using 10356-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of human kidney using 10356-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse adipose tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse adipose using 10356-1-AP

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human colon cancer using 10356-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human stomach cancer using 10356-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    IF Staining of HeLa using 10356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse adipose tissue, human kidney tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10356-1-AP targets GGA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGA2 fusion protein Ag0370 相同性解析による交差性が予測される生物種
Full Name golgi associated, gamma adaptin ear containing, ARF binding protein 2
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC000284
Gene symbol GGA2
Gene ID (NCBI) 23062
RRIDAB_2110735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Protocols

Product Specific Protocols
WB protocol for GGA2 antibody 10356-1-APDownload protocol
IHC protocol for GGA2 antibody 10356-1-APDownload protocol
IF protocol for GGA2 antibody 10356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.

Authors - Arunas Kvainickas
View Article
ratWB

Front Cardiovasc Med

Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy.

Authors - Bing Li
View Article