Validation Data Gallery
Tested Applications
| Positive WB detected in | human stomach tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
Product Information
13309-1-AP targets GHRL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GHRL fusion protein Ag4141 相同性解析による交差性が予測される生物種 |
| Full Name | ghrelin/obestatin prepropeptide |
| Calculated molecular weight | 117 aa, 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC025791 |
| Gene Symbol | GHRL |
| Gene ID (NCBI) | 51738 |
| RRID | AB_2294726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBU3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is predominantly produced in endocrine cells in the gastric mucosa, called X/A-like or ghrelin cells, from where it is secreted into the plasma. In the pituitary gland, ghrelin stimulates GH release and regulates food intake and energy metabolism. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GHRL antibody 13309-1-AP | Download protocol |
| IHC protocol for GHRL antibody 13309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms. | ||
Pancreas Effects of Ghrelin miRNA on Inflammation and Calcium Pathway in Pancreatic Acinar Cells of Acute Pancreatitis | ||
Biosci Rep Differential expression of ghrelin and GHSR via the mTOR pathway during the dynamic carcinogenic process involving oral, potentially malignant disorders. | ||
Neuroreport Expression of ghrelin or growth hormone secretagogue receptor in the brain of postpartum stress mice. | ||
Hepatobiliary Pancreat Dis Int Ghrelin inhibits IKKβ/NF-κB activation and reduces pro-inflammatory cytokine production in pancreatic acinar AR42J cells treated with cerulein. | ||
Eur J Nutr Improvement of functional dyspepsia with Suaeda salsa (L.) Pall via regulating brain-gut peptide and gut microbiota structure |