VISTA Polyclonal antibody

Name: VISTA Polyclonal antibody
Brand: Proteintech
SKU: 24849-1-AP

VISTA Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24849-1-AP

Synonyms

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

Validation Data Gallery

Filter:

AllWBIHCIF-P

WB analysis using 24849-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 24849-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24849-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 24849-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human placenta using 24849-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 24849-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24849-1-AP (VISTA rabbit polyclonal antibody) at dilution of 1:100 and 60181-1-Ig (CD3 mouse monoclonal antibody), further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 24849-1-AP and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60181-1-Ig.
IF Staining of human placenta using 24849-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 24849-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human placenta tissue, SH-SY5Y cells, human peripheral blood platelets
Positive IHC detected in	human placenta tissue, human colon cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human placenta tissue, human tonsillitis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

24849-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	VISTA fusion protein Ag20600 相同性解析による交差性が予測される生物種
Full Name	chromosome 10 open reading frame 54
Calculated molecular weight	311 aa, 34 kDa
Observed molecular weight	45-60 kDa
GenBank accession number	BC020568
Gene symbol	VISTA
Gene ID (NCBI)	64115
RRID	AB_2879755
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
WB protocol for VISTA antibody 24849-1-AP	Download protocol
IHC protocol for VISTA antibody 24849-1-AP	Download protocol
IF protocol for VISTA antibody 24849-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Nutr Biochem Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression Authors - Qi Zhang View Article
human	IF	Front Cell Dev Biol Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis. Authors - Yuanyuan Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VISTA Polyclonal antibody

VISTA Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 24849-1-AP

WB analysis of SH-SY5Y using 24849-1-AP

IHC staining of human placenta using 24849-1-AP

IHC staining of human placenta using 24849-1-AP

IHC staining of human tonsillitis using 24849-1-AP

IHC staining of human tonsillitis using 24849-1-AP

IHC staining of human colon cancer using 24849-1-AP

IHC staining of human colon cancer using 24849-1-AP

IF Staining of human placenta using 24849-1-AP

IF Staining of human placenta using 24849-1-AP

IF Staining of human tonsillitis using 24849-1-AP

IF Staining of human placenta using 24849-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis.