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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GILZ Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 3.13-200 ng/mL

Cat No : MP00887-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00887-3, Human GILZ Recombinant Matched Antibody Pair - PBS only. 83967-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3015. 83967-4-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

    Sandwich ELISA standard curve of MP00887-3

    Sandwich ELISA standard curve of MP00887-3, Human GILZ Recombinant Matched Antibody Pair - PBS only. 83967-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3015. 83967-4-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • The mean GILZ concentration was determined to be 596.32 ng/mL in HEK-293T cell extract based on a 3.2 mg/mL extract load.

    Sample test of MP00887-3

    The mean GILZ concentration was determined to be 596.32 ng/mL in HEK-293T cell extract based on a 3.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00887-3 targets GILZ in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 3.13-200 ng/mL (Sandwich ELISA);

Capture antibody: TSC22D3 Recombinant antibody, PBS Only (Capture) 83967-5-PBS (241024D5). 100 μg. Concentration 1 mg/ml.

Detection antibody: GILZ Recombinant antibody, PBS Only (Detector) 83967-4-PBS (241024F5). 100 μg. Concentration 1 mg/ml.

Alternative GILZ matched antibody pairs: MP00887-1, MP00887-2

Matched pair protein standard: Ag3015

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83967-5-PBS
Tested Reactivity human
Immunogen TSC22D3 fusion protein Ag3015
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TSC22 domain family, member 3
Synonyms GILZ, TSC 22R, DSIP-immunoreactive peptide, DSIPI, Delta sleep-inducing peptide immunoreactor
GenBank accession numberBC018148
Gene symbol GILZ
UniprotQ99576
Gene ID (NCBI) 1831
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83967-4-PBS
Tested Reactivity human
Immunogen GILZ fusion protein Ag3015
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TSC22 domain family, member 3
Synonyms TSC22 D3, TSC 22R, DSIP-immunoreactive peptide, DSIPI, Delta sleep-inducing peptide immunoreactor
GenBank accession numberBC018148
Gene symbol GILZ
UniprotQ99576
Gene ID (NCBI) 1831
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.