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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GIMAP2 Polyclonal antibody

GIMAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13445-1-AP

Synonyms

GIMAP2, GTPase IMAP family member 2, GTPase, IMAP family member 2, HIMAP2, IMAP2, Immunity associated protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using GIMAP2 Polyclonal antibody (13445-1-AP)

    WB analysis of human heart using 13445-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13445-1-AP (GIMAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using GIMAP2 Polyclonal antibody (13445-1-AP)

    WB analysis of human kidney using 13445-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13445-1-AP (GIMAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using GIMAP2 Polyclonal antibody (13445-1-AP)

    IHC staining of human heart using 13445-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GIMAP2 Polyclonal antibody (13445-1-AP)

    IHC staining of human heart using 13445-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inhuman heart tissue, human kidney tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13445-1-AP targets GIMAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIMAP2 fusion protein Ag4273 相同性解析による交差性が予測される生物種
Full Name GTPase, IMAP family member 2
Calculated molecular weight 337 aa, 38 kDa
Observed molecular weight 28-40 kDa
GenBank accession numberBC032345
Gene symbol GIMAP2
Gene ID (NCBI) 26157
RRIDAB_2111871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This protein was not eliminated clearly. It belongs to GTPase of the immunity-associated protein family. One of its homologous protein, GIMAP1, runs as a 30kd protein in SDS-PAGE, lower than the calculated molecular weight. This antibody detected one 28kd band in several tissues.

Protocols

Product Specific Protocols
WB protocol for GIMAP2 antibody 13445-1-APDownload protocol
IHC protocol for GIMAP2 antibody 13445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols