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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GIMAP4 Polyclonal antibody

GIMAP4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11852-1-AP

Synonyms

Immunity associated protein 4, IMAP4, IAN-1, IAN1, IAN 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GIMAP4 Polyclonal antibody (11852-1-AP)

    WB analysis of Jurkat using 11852-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11852-1-AP (GIMAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GIMAP4 Polyclonal antibody (11852-1-AP)

    IP experiment of Jurkat using 11852-1-AP

    IP result of anti-GIMAP4 (IP:11852-1-AP, 3ug; Detection:11852-1-AP 1:800) with Jurkat cells lysate 1080ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GIMAP4 Polyclonal antibody (11852-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11852-1-AP (GIMAP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11852-1-AP targets GIMAP4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIMAP4 fusion protein Ag2422 相同性解析による交差性が予測される生物種
Full Name GTPase, IMAP family member 4
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC020657
Gene symbol GIMAP4
Gene ID (NCBI) 55303
RRIDAB_2279105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis. The MW of this protein is 38 kDa, and Catalog#11852-1-AP specially recognises the 38 kDa protein.

Protocols

Product Specific Protocols
WB protocol for GIMAP4 antibody 11852-1-APDownload protocol
IHC protocol for GIMAP4 antibody 11852-1-APDownload protocol
IP protocol for GIMAP4 antibody 11852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols