GIMAP5 Polyclonal antibody
GIMAP5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11068-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
11068-1-AP targets GIMAP5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GIMAP5 fusion protein Ag1509 相同性解析による交差性が予測される生物種 |
| Full Name | GTPase, IMAP family member 5 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC011732 |
| Gene symbol | GIMAP5 |
| Gene ID (NCBI) | 55340 |
| RRID | AB_2111880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GIMAP5 gene encodes immunity-associated nucleotide 5 protein (IAN5) belonging to GTPase immune-associated protein subfamily. GIMAP5 is widely expressed with high levels in lymph node and spleen. The loss of Gimap5 (GTPase of the 5) gene function is the underlying cause of lymphopenia and autoimmune diabetes in the BioBreeding (BB) rat. Mouse gimap5 is necessary for the survival of peripheral T cells, NK/NKT-cell development, hematopoietic progenitor cell survival and the maintenance of normal liver function. GIMAP5 may regulate T lymphocyte survival by mechanisms that operate upstream of mitochondria.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GIMAP5 antibody 11068-1-AP | Download protocol |
| IHC protocol for GIMAP5 antibody 11068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood Cells Mol Dis Notch protection against apoptosis in T-ALL cells mediated by GIMAP5. |
