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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GIMAP7 Monoclonal antibody (68451-1-Ig)

    WB analysis using 68451-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68451-1-Ig (GIMAP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GIMAP7 Monoclonal antibody (68451-1-Ig)

    IP experiment of Jurkat using 68451-1-Ig

    IP result of anti-GIMAP7 (IP:68451-1-Ig, 4ug; Detection:68451-1-Ig 1:2000) with Jurkat cells lysate 1560 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GIMAP7 Monoclonal antibody (68451-1-Ig)

    IF Staining of HepG2 using 68451-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GIMAP7 antibody (68451-1-Ig, Clone: 3E4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GIMAP7 Monoclonal antibody

GIMAP7 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68451-1-Ig
Clone No.3E4A6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

GIMAP7, GTPase IMAP family member 7, GTPase, IMAP family member 7, hIAN7, IAN 7, IAN7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNK-92 cells, Jurkat cells, MOLT-4 cells, HUVEC cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68451-1-Ig targets GIMAP7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GIMAP7 fusion protein Ag10740 相同性解析による交差性が予測される生物種
Full Name GTPase, IMAP family member 7
Calculated molecular weight 300 aa, 35 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC027613
Gene Symbol GIMAP7
Gene ID (NCBI) 168537
RRIDAB_3085163
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NHV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GIMAP7, also known as hIAN7 or IAN 7, is a transmembrane protein located in the endoplasmic reticulum and Golgi apparatus. GIMAP7 can promote oxidative stress and apoptosis in ovarian granulosa cells in Polycystic ovary syndrome by inhibiting the SHH signalling pathway(PMID: 36581994). The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

Protocols

Product Specific Protocols
WB protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IF protocol for GIMAP7 antibody 68451-1-IgDownload protocol
IP protocol for GIMAP7 antibody 68451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols