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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GINS1 Polyclonal antibody (16061-1-AP)

    WB analysis using 16061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GINS1 Polyclonal antibody (16061-1-AP)

    WB analysis using 16061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GINS1 Polyclonal antibody (16061-1-AP)

    IHC staining of human ovary cancer using 16061-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16061-1-AP (GINS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GINS1 Polyclonal antibody

GINS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16061-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PSF1, KIAA0186, GINS complex subunit 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, mouse testis tissue, SH-SY5Y cells, U-251 cells, mouse thymus tissue, rat testis tissue, rat thymus tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16061-1-AP targets GINS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GINS1 fusion protein Ag8895 相同性解析による交差性が予測される生物種
Full Name GINS complex subunit 1 (Psf1 homolog)
Calculated molecular weight 196 aa, 23 kDa
Observed molecular weight20 kDa
GenBank accession numberBC012542
Gene Symbol GINS1
Gene ID (NCBI) 9837
RRIDAB_3669229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14691
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GINS1, also known as Partner of SLD Five 1 (PSF1) is a member of the GINS (Go-Ichi-Nii-San) complex, which plays a vital role in the initiation and elongation of DNA replication (PMID: 34414190). GINS1 was initially found to be predominantly expressed in highly proliferating cells instead of mature cells. Later on, it was found that GINS was involved in the tumorigenesis of various cancers, including breast cancer, colorectal cancer, and hepatocellular carcinoma (PMID: 36065190).

Protocols

Product Specific Protocols
WB protocol for GINS1 antibody 16061-1-APDownload protocol
IHC protocol for GINS1 antibody 16061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols