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- KD/KO Validated
PSF2 Polyclonal antibody
PSF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 16247-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
16247-1-AP targets PSF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSF2 fusion protein Ag9180 相同性解析による交差性が予測される生物種 |
| Full Name | GINS complex subunit 2 (Psf2 homolog) |
| Calculated molecular weight | 185 aa, 21 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC010164 |
| Gene symbol | GINS2 |
| Gene ID (NCBI) | 51659 |
| RRID | AB_2111895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSF2 antibody 16247-1-AP | Download protocol |
| IHC protocol for PSF2 antibody 16247-1-AP | Download protocol |
| IF protocol for PSF2 antibody 16247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
J Cell Biol LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.
| ||
Cell Rep Impaired p32 Regulation Caused by the Lymphoma-Prone RECQ4 Mutation Drives Mitochondrial Dysfunction. | ||
Oncogene Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality. | ||
J Biol Chem TIMELESS suppresses the accumulation of aberrant CDC45-MCM2-7-GINS replicative helicase complexes on human chromatin. |
