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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSF3 Polyclonal antibody

PSF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16345-1-AP

Synonyms

GINS3, GINS complex subunit 3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PSF3 Polyclonal antibody (16345-1-AP)

    WB analysis of MCF-7 using 16345-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16345-1-AP (GINS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSF3 Polyclonal antibody (16345-1-AP)

    WB analysis of HeLa cells using 16345-1-AP

    WB result from Andre Franz in Hela cell of 16345-1-AP (anti-GINS3).

  • Western Blot (WB) analysis of MCF-7 cells using PSF3 Polyclonal antibody (16345-1-AP)

    WB analysis of MCF-7 using 16345-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16345-1-AP (GINS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PSF3 Polyclonal antibody (16345-1-AP)

    IHC staining of human ovary cancer using 16345-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16345-1-AP (PSF3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSF3 Polyclonal antibody (16345-1-AP)

    IF Staining of HeLa using 16345-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16345-1-AP (GINS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16345-1-AP targets PSF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSF3 fusion protein Ag9534 相同性解析による交差性が予測される生物種
Full Name GINS complex subunit 3 (Psf3 homolog)
Calculated molecular weight 216 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC014437
Gene symbol GINS3
Gene ID (NCBI) 64785
RRIDAB_10804660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSF3 antibody 16345-1-APDownload protocol
IHC protocol for PSF3 antibody 16345-1-APDownload protocol
IF protocol for PSF3 antibody 16345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols