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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GJB6 Polyclonal antibody

GJB6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24215-1-AP

Synonyms

Connexin 30, CX30, DFNA3, DFNA3B, DFNB1B, ED2, EDH, Gap junction beta 6 protein, GJB6, HED

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GJB6 Polyclonal antibody (24215-1-AP)

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GJB6 Polyclonal antibody (24215-1-AP)

    IHC staining of human oesophagus using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GJB6 Polyclonal antibody (24215-1-AP)

    IHC staining of human skin cancer using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GJB6 Polyclonal antibody (24215-1-AP)

    IHC staining of human skin cancer using 24215-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24215-1-AP (GJB6 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman oesophagus tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24215-1-AP is GJB6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GJB6 fusion protein Ag21529 相同性解析による交差性が予測される生物種
Full Name gap junction protein, beta 6, 30kDa
Calculated molecular weight 261 aa, 30 kDa
GenBank accession numberBC038934
Gene symbol GJB6
Gene ID (NCBI) 10804
RRIDAB_2879461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gap junctions form conduits between adjacent cells that are composed of connexin protein subunits and provide direct intercellular communication pathways allowing rapid exchange of ions and metabolites (PMID: 12126230; 15094343). Connexins are four-pass transmembrane proteins with amino- and carboxy-terminal regions facing the cytoplasm. A connexon is composed of a hexamer of connexins. GJB6 (gap junction beta-6 protein, also known as connexin-30) is a member of the connexin family of proteins. GJB6 is a component of the gap junction networks of the cochlea.

Protocols

Product Specific Protocols
IHC protocol for GJB6 antibody 24215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols