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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GJC2 Polyclonal antibody (55376-1-AP)

    WB analysis of mouse brain using 55376-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55376-1-AP (GJC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using GJC2 Polyclonal antibody (55376-1-AP)

    IHC staining of human cerebellum using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GJC2 Polyclonal antibody (55376-1-AP)

    IHC staining of human brain using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using GJC2 Polyclonal antibody (55376-1-AP)

    IHC staining of human brain using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using GJC2 Polyclonal antibody (55376-1-AP)

    IHC staining of human cerebellum using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GJC2 Polyclonal antibody

GJC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55376-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Connexin 46.6, Connexin 47, CX46.6, Cx47, Gap junction alpha 12 protein, Gap junction gamma 2 protein, GJA12, GJC2, HLD2, PMLDAR, SPG44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55376-1-AP targets GJC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name gap junction protein, gamma 2, 47kDa
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_020435
Gene Symbol GJC2
Gene ID (NCBI) 57165
RRIDAB_2881317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GJC2 antibody 55376-1-APDownload protocol
IHC protocol for GJC2 antibody 55376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols