Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of HepG2 using 13360-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 13360-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13360-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13360-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 13360-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 13360-1-AP
IP result of anti-Glycerokinase (IP:13360-1-AP, 4ug; Detection:13360-1-AP 1:1000) with HepG2 cells lysate 4000ug.
IF Staining of HepG2 using 13360-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glycerokinase antibody (13360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 13360-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glycerokinase antibody (13360-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Glycerokinase Polyclonal antibody

Glycerokinase Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 13360-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

GK, GKD, GK1, EC:2.7.1.30, ATP:glycerol 3-phosphotransferase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse kidney tissue, mouse liver tissue, mouse testis tissue
Positive IP detected in	HepG2 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 2 publications below
IF	See 1 publications below
RIP	See 1 publications below

Product Information

13360-1-AP targets Glycerokinase in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Glycerokinase fusion protein Ag4175 相同性解析による交差性が予測される生物種
Full Name	glycerol kinase
Calculated molecular weight	524 aa, 57 kDa
Observed molecular weight	60-65 kDa
GenBank accession number	BC037549
Gene Symbol	Glycerokinase
Gene ID (NCBI)	2710
RRID	AB_1167391
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P32189
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Glycerokinase antibody 13360-1-AP	Download protocol
IF protocol for Glycerokinase antibody 13360-1-AP	Download protocol
IP protocol for Glycerokinase antibody 13360-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis Authors - Xu Yang View Article
pig	WB	Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. Authors - Yongjie Xu View Article
mouse	WB	J Appl Toxicol Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice. Authors - Ni Zeng View Article
human	WB	Life Sci Alliance Serine palmitoyltransferase assembles at ER-mitochondria contact sites. Authors - Mari J Aaltonen View Article
human,mouse	IHC	J Adv Res O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation. Authors - Hongshuo Zhang View Article
human,mouse	WB,IHC	Mol Metab The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326 Authors - Brian E Ford View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 13360-1-AP

WB analysis of mouse liver using 13360-1-AP

WB analysis of HepG2 using 13360-1-AP

WB analysis of mouse kidney using 13360-1-AP

WB analysis of mouse testis using 13360-1-AP

IP experiment of HepG2 using 13360-1-AP

IF Staining of HepG2 using 13360-1-AP

IF Staining of HepG2 using 13360-1-AP

Glycerokinase Polyclonal antibody

Glycerokinase Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

Aberrant expression of miR-451a contributes to 1,2-dichloroethane-induced hepatic glycerol gluconeogenesis disorder by inhibiting glycerol kinase expression in NIH Swiss mice.

Serine palmitoyltransferase assembles at ER-mitochondria contact sites.

O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.

The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326