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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GK3P Polyclonal antibody

GK3P Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14176-1-AP

Synonyms

GK3P, GKP3, GKTB, glycerol kinase 3 pseudogene

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GK3P Polyclonal antibody (14176-1-AP)

    WB analysis of HeLa using 14176-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using GK3P Polyclonal antibody (14176-1-AP)

    WB analysis of mouse testis using 14176-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GK3P Polyclonal antibody (14176-1-AP)

    IHC staining of human skin cancer using 14176-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GK3P Polyclonal antibody (14176-1-AP)

    IHC staining of human skin cancer using 14176-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14176-1-AP targets GK3P in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GK3P fusion protein Ag5383 相同性解析による交差性が予測される生物種
Full Name glycerol kinase 3 pseudogene
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC066960
Gene symbol GK3P
Gene ID (NCBI) 2713
RRIDAB_1288669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GK3P antibody 14176-1-APDownload protocol
IHC protocol for GK3P antibody 14176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols