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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha galactosidase A Polyclonal antibody

Alpha galactosidase A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19877-1-AP

Synonyms

GLA, Galactosylgalactosylglucosylceramidase GLA, GALA, EC:3.2.1.22, Alpha-galactosidase A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using Alpha galactosidase A Polyclonal antibody (19877-1-AP)

    WB analysis of HEK-293 using 19877-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19877-1-AP (Alpha galactosidase A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Alpha galactosidase A Polyclonal antibody (19877-1-AP)

    WB analysis of HEK-293 using 19877-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19877-1-AP (Alpha galactosidase A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha galactosidase A Polyclonal antibody (19877-1-AP)

    IF Staining of HepG2 using 19877-1-AP

    Immunofluorescent analysis of HepG2 cells, using GLA antibody 19877-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19877-1-AP targets Alpha galactosidase A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha galactosidase A fusion protein Ag7505 相同性解析による交差性が予測される生物種
Full Name galactosidase, alpha
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002689
Gene symbol GLA
Gene ID (NCBI) 2717
RRIDAB_10642955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLA, also named as Melibiase, Agalsidas and Alpha-galactosidase A, Belongs to the glycosyl hydrolase 27 family. It hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Protocols

Product Specific Protocols
WB protocol for Alpha galactosidase A antibody 19877-1-APDownload protocol
IF protocol for Alpha galactosidase A antibody 19877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Septin4 promotes cell death in human colon cancer cells by interacting with BAX.

Authors - Xin Zhao
View Article