Back

Beta Galactosidase 抗体 17 文献

Rabbit Polyclonal| カタログ番号: 15518-1-AP

  • Print page
  • Download PDF

--

（希望販売価格）

販売および在庫状況はコスモ・バイオ（株）にお問い合わせください。
distributor

適用アプリケーション

アプリケーション:
WB, IHC, IF
Application not tested? Please see our Guarantee


Images See all

Beta Galactosidase Antibody WB SH-SY5Y cells 15518-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Beta Galactosidase Antibody IF HeLa cells 15518-1-AP

Immunofluorescent analysis of Hela cells, using GLB1 antibody 15518-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Beta Galactosidase Antibody IHC human gliomas tissue 15518-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Beta Galactosidase Antibody IHC human gliomas tissue 15518-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

種特異性:
human, mouse, rat
WB ポジティブ:
SH-SY5Y cells
IHC ポジティブ:
human gliomas tissue, human liver cancer tissue
IF ポジティブ:
Hela cells
推奨希釈倍率:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Beta Galactosidase fusion protein Ag7792
正式名:
galactosidase, beta 1
計算分子量:
76 kDa
検出分子量:
67 kDa
GenBank accession number:
BC007493
Gene ID (NCBI):
2720
遺伝子シンボル:
GLB1
別名:
Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

背景説明

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.


Back
to top