GLI1 Polyclonal antibody

GLI1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25733-1-AP

Synonyms

GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1


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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25733-1-AP is GLI1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLI1 fusion protein Ag22962 相同性解析による交差性が予測される生物種
Full Name GLI family zinc finger 1
Calculated molecular weight 1106 aa, 118 kDa
GenBank accession numberBC013000
Gene symbol GLI1
Gene ID (NCBI) 2735
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanWB,IHC

J Orthop Translat

RPL35 downregulated by mechanical overloading promotes chondrocyte senescence and osteoarthritis development via Hedgehog-Gli1 signaling

Authors - Jinjian Zhu
humanWB

Lab Chip

Paper-based microreactor array for rapid screening of cell signaling cascades.

Authors - Chia-Hao Huang
humanIF

Oncotarget

Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide.

Authors - Jilian R Melamed
humanWB

Hum Cell

Retraction Note: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis

Authors - Fan Li
humanWB

Oncol Rep

Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.

Authors - Jingmin Li
humanWB

Am J Transl Res

Involvement of hedgehog signaling in all-trans retinoic acid-mediated suppression of colon cancer

Authors - Yu Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of weak nuclear and punctate cytosolic.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: U87 Human Glioblastoma
GLI1 Antibody Immunofluorescence, validation (1:500 dilution) in U87 Human Glioblastoma (Cat no:25733-1-AP)