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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis of C6 using 66905-1-Ig (same clone as 66905-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLI1 Monoclonal antibody (66905-1-Ig)

    WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GLI1 Monoclonal antibody (66905-1-Ig)

    IF Staining of HeLa using 66905-1-Ig (same clone as 66905-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLI1 antibody (66905-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GLI1 Monoclonal antibody, PBS Only

GLI1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66905-1-PBS
Clone No.1D2B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Oncogene GLI, Glioma-associated oncogene, Glioma associated oncogene, GLI family zinc finger 1, GLI

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66905-1-PBS targets GLI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GLI1 fusion protein Ag27832 相同性解析による交差性が予測される生物種
Full Name GLI family zinc finger 1
Calculated molecular weight 1106 aa, 118 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC013000
Gene Symbol GLI1
Gene ID (NCBI) 2735
RRIDAB_2882232
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08151
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.