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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLI2 Polyclonal antibody

GLI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28245-1-AP

Synonyms

THP2, THP1, THP, Tax helper protein, HPE9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLI2 Polyclonal antibody (28245-1-AP)

    WB analysis using 28245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28245-1-AP (GLI2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GLI2 Polyclonal antibody (28245-1-AP)

    IHC staining of human breast cancer using 28245-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28245-1-AP (GLI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GLI2 Polyclonal antibody (28245-1-AP)

    IHC staining of human breast cancer using 28245-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28245-1-AP (GLI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, U2OS cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28245-1-AP targets GLI2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLI2 fusion protein Ag28422 相同性解析による交差性が予測される生物種
Full Name GLI family zinc finger 2
Calculated molecular weight 168 aa
Observed molecular weight180-200 kDa
GenBank accession numberNM_005270
Gene symbol GLI2
Gene ID (NCBI) 2736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLI2, along with the related GLI1 and GLI3, is a member of the GLI family of transcription factors belonging to the Glis superfamily. Glioma-Associated Oncogene Family Zinc Finger 2 (GLI2) is a zinc-finger transcription factor involved in the Sonic Hedgehog pathway. GLI2 is involved in proliferation, invasion, fibrosis, and is involved in carcinogenesis in various malignancies including gastric cancer, lung cancer, prostate cancer, bladder cancer, and so on.

Protocols

Product Specific Protocols
WB protocol for GLI2 antibody 28245-1-APDownload protocol
IHC protocol for GLI2 antibody 28245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols