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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLI2-Specific Recombinant antibody, PBS Only

GLI2-Specific Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241049C10

Cat no : 84141-1-PBS

Synonyms

Gli2, Tax helper protein, GLI Kruppel family member GLI2, Gli 2, AW546128

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLI2-Specific Recombinant antibody (84141-1-RR)

    WB analysis using 84141-1-RR (same clone as 84141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84141-1-RR (GLI2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GLI2-Specific Recombinant antibody (84141-1-RR)

    IF Staining of HepG2 using 84141-1-RR (same clone as 84141-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GLI2-Specific antibody (84141-1-RR, Clone: 241049C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84141-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84141-1-PBS targets GLI2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GLI family zinc finger 2
Calculated molecular weight 168 kDa
Observed molecular weight80-90 kDa, 130-140 kDa, 160-180 kDa
GenBank accession numberNM_005270
Gene symbol GLI2
Gene ID (NCBI) 2736
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.