GLI3-Specific Polyclonal antibody

Name: GLI3-Specific Polyclonal antibody
Brand: Proteintech
SKU: 19949-1-AP

GLI3-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19949-1-AP

Synonyms

ACLS, GCPS, GLI family zinc finger 3, GLI3, GLI3-Specific, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3

Validation Data Gallery

Filter:

AllWBIPIHCIF

WB analysis of human placenta using 19949-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 19949-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 19949-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19949-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19949-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 19949-1-AP
IP result of anti-GLI3-Specific (IP:19949-1-AP, 4ug; Detection:19949-1-AP 1:300) with mouse lung tissue lysate 4000ug.
IHC staining of human testis using 19949-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.
IHC staining of human colon using 19949-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.
IHC staining of human colon using 19949-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.
IHC staining of human testis using 19949-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.
IF Staining of HepG2 using 19949-1-AP
Immunofluorescent analysis of HepG2 cells using 19949-1-AP (GLI3-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

-/ -

Cat No. 19949-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human placenta tissue, mouse lung tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human testis tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below

Product Information

19949-1-AP targets GLI3-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	GLI family zinc finger 3
Calculated molecular weight	170 kDa
Observed molecular weight	190 kDa, 83-86 kDa
GenBank accession number	NM_000168
Gene symbol	GLI3
Gene ID (NCBI)	2737
RRID	AB_11182281
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Protocols

Product Specific Protocols
WB protocol for GLI3-Specific antibody 19949-1-AP	Download protocol
IHC protocol for GLI3-Specific antibody 19949-1-AP	Download protocol
IF protocol for GLI3-Specific antibody 19949-1-AP	Download protocol
IP protocol for GLI3-Specific antibody 19949-1-AP	Download protocol
FC protocol for GLI3-Specific antibody 19949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Authors - Bo Yan View Article
mouse	WB	JCI Insight Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling. Authors - Hairong Shi View Article
mouse	WB	Acta Pharmacol Sin 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia Authors - Xiao-Wei Li View Article
mouse	WB	PLoS Genet Depletion of Ric-8B leads to reduced mTORC2 activity. Authors - Maíra H Nagai View Article
mouse	WB	Sci Rep LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration. Authors - Yuqin Men View Article
mouse	WB	Int J Biol Sci MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types. Authors - Xiaolin Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLI3-Specific Polyclonal antibody

GLI3-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis of human placenta using 19949-1-AP

WB analysis of human placenta using 19949-1-AP

WB analysis of human placenta using 19949-1-AP

WB analysis of mouse lung using 19949-1-AP

WB analysis of mouse lung using 19949-1-AP

IP experiment of mouse lung using 19949-1-AP

IHC staining of human testis using 19949-1-AP

IHC staining of human colon using 19949-1-AP

IHC staining of human colon using 19949-1-AP

IHC staining of human testis using 19949-1-AP

IF Staining of HepG2 using 19949-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.

1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia

Depletion of Ric-8B leads to reduced mTORC2 activity.

LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration.

MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.