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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLIPR1L1 Recombinant antibody

GLIPR1L1 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240594D10

Cat no : 83584-3-RR

Synonyms

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using GLIPR1L1 Recombinant antibody (83584-3-RR)

    WB analysis of human testis using 83584-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using GLIPR1L1 Recombinant antibody (83584-3-RR)

    WB analysis of mouse testis using 83584-3-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using GLIPR1L1 Recombinant antibody (83584-3-RR)

    FC experiment of PC-3 using 83584-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83584-3-RR

    Affinity and Kinetic Characterization of 83584-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

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Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83584-3-RR is GLIPR1L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GLIPR1L1 fusion protein Ag14852 相同性解析による交差性が予測される生物種
Full Name GLI pathogenesis-related 1 like 1
Calculated molecular weight 242 aa, 27 kDa
Observed molecular weight25 kDa
GenBank accession numberBC014603
Gene symbol GLIPR1L1
Gene ID (NCBI) 256710
RRIDAB_3671198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GLIPR1L1 antibody 83584-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols