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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of mouse kidney using 12678-1-AP (same clone as 12678-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of A549 using 12678-1-AP (same clone as 12678-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of BxPC-3 using 12678-1-AP (same clone as 12678-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of HepG2 using 12678-1-AP (same clone as 12678-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

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GLIS3 Polyclonal antibody, PBS Only

GLIS3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12678-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12678-1-PBS targets GLIS3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLIS3 fusion protein Ag3366 相同性解析による交差性が予測される生物種
Full Name GLIS family zinc finger 3
Calculated molecular weight 775 aa, 84 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC033899
Gene Symbol GLIS3
Gene ID (NCBI) 169792
RRIDAB_2877873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEA6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. GLIS3 (gLIS family zinc finger 3), also known as ZNF515 that localizes to the nucleus and contains five C2H2-type zinc fingers. Expressed in a variety of tissues, including kidney, brain, liver, lung, ovary, pancreas, thymus and skeletal muscle, GLIS3 functions as both an activator and a suppressor of transcription, specifically binding the consensus sequence 5'-GACCACCCAC-3' through its C2H2-type zinc fingers. Defects in the gene encoding GLIS3 are a cause of NDH syndrome; a neonatal diabetes that is characterized by congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. There are various isoform of GLIS3 and molecular weight of one isoform is 70 kDa.