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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GLO1 Polyclonal antibody (18477-1-AP)

    WB analysis of HeLa using 18477-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18477-1-AP (GLO1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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GLO1 Polyclonal antibody

GLO1 Polyclonal Antibody for WB, ELISA
Cat No. 18477-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18477-1-AP targets GLO1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLO1 fusion protein Ag13386 相同性解析による交差性が予測される生物種
Full Name glyoxalase I
Calculated molecular weight 21 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC001741
Gene Symbol GLO1
Gene ID (NCBI) 2739
RRIDAB_10640585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GLO1 antibody 18477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols