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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLOD4 Polyclonal antibody

GLOD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16788-1-AP

Synonyms

Glyoxalase domain-containing protein 4, GLOD 4, CGI-150, CGI150, CGI 150

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GLOD4 Polyclonal antibody (16788-1-AP)

    WB analysis using 16788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    WB analysis of mouse kidney using 16788-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    WB analysis of human placenta using 16788-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of mouse ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of human heart using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of human heart using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of mouse ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of human ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GLOD4 Polyclonal antibody (16788-1-AP)

    IHC staining of human ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, human placenta tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, mouse ovary tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16788-1-AP targets GLOD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymonkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLOD4 fusion protein Ag10222 相同性解析による交差性が予測される生物種
Full Name glyoxalase domain containing 4
Calculated molecular weight 289 aa, 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008605
Gene symbol GLOD4
Gene ID (NCBI) 51031
RRIDAB_2247517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GLOD4 antibody 16788-1-APDownload protocol
IHC protocol for GLOD4 antibody 16788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyIHC

Mol Biosyst

Unravelling the proteome of adult rhesus monkey ovaries.

Authors - Hui He
View Article