Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse ovary tissue |
| Positive IHC detected in | human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
Product Information
55292-1-AP targets ZGLP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | GATA like protein-1 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | NM_001103167 |
| Gene Symbol | GLP-1 |
| Gene ID (NCBI) | 100125288 |
| RRID | AB_2881301 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0C6A0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZGLP1 antibody 55292-1-AP | Download protocol |
| IHC protocol for ZGLP1 antibody 55292-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chin J Integr Med Huanglian-Renshen-Decoction Maintains Islet β-Cell Identity in T2DM Mice through Regulating GLP-1 and GLP-1R in Both Islet and Intestine | ||
Front Pharmacol Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell |