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  • Featured Product
  • KD/KO Validated

KGA/GAC Recombinant antibody, PBS Only

KGA/GAC Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C20

Cat no : 81486-1-PBS

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using KGA/GAC Recombinant antibody (81486-1-RR)

    WB analysis of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    WB result of GLS antibody (81486-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLS transfected A549 cells. This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KGA/GAC Recombinant antibody (81486-1-RR)

    WB analysis using 81486-1-RR (same clone as 81486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KGA/GAC Recombinant antibody (81486-1-RR)

    IHC staining of mouse brain using 81486-1-RR (same clone as 81486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81486-1-RR (GLS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KGA/GAC Recombinant antibody (81486-1-RR)

    IF Staining of A549 using 81486-1-RR (same clone as 81486-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GLS antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81486-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81486-1-RR

    Affinity and Kinetic Characterization of 81486-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81486-1-RR against Human GLS were performed. The affinity constant is 0.182 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81486-1-PBS targets KGA/GAC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KGA/GAC fusion protein Ag3505 相同性解析による交差性が予測される生物種
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight58 kDa, 65 kDa
GenBank accession numberBC038507
Gene symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.