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KD/KO Validated

GLT25D1 Polyclonal antibody

Name: GLT25D1 Polyclonal antibody
Brand: Proteintech
SKU: 16768-1-AP

GLT25D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16768-1-AP

Synonyms

PSEC0241, EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 1, COLGALT1, ColGalT 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of SH-SY5Y using 16768-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 16768-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 16768-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IP experiment of HeLa using 16768-1-AP
IP result of anti-GLT25D1 (IP:16768-1-AP, 4ug; Detection:16768-1-AP 1:500) with HeLa cells lysate 4000ug.
IHC staining of human ovary tumor using 16768-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse placenta using 16768-1-AP
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SH-SY5Y using 16768-1-AP
Immunofluorescent analysis of SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SH-SY5Y using 16768-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 16768-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SH-SY5Y cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, mouse placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

16768-1-AP targets GLT25D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GLT25D1 fusion protein Ag10346 相同性解析による交差性が予測される生物種
Full Name	glycosyltransferase 25 domain containing 1
Calculated molecular weight	231 aa, 27 kDa
Observed molecular weight	72 kDa
GenBank accession number	BC020492
Gene symbol	GLT25D1
Gene ID (NCBI)	79709
RRID	AB_2878313
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GLT25D1 antibody 16768-1-AP	Download protocol
IHC protocol for GLT25D1 antibody 16768-1-AP	Download protocol
IF protocol for GLT25D1 antibody 16768-1-AP	Download protocol
IP protocol for GLT25D1 antibody 16768-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Ann Neurol Biallelic COLGALT1 variants are associated with cerebral small vessel disease. Authors - Satoko Miyatake View Article
mouse,rat	WB	J Mol Cell Cardiol Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion. Authors - Randy T Cowling View Article
mouse	WB	PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. Authors - Masahiko Terajima View Article
mouse	WB	Sci Rep Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes Authors - Masahiko Terajima View Article
human	WB	Biochemistry Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes. Authors - Masahiko Terajima KD Validated View Article
mouse	WB,IHC	Mol Med Rep Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway. Authors - Xiaohui Ye KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLT25D1 Polyclonal antibody

GLT25D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of SH-SY5Y using 16768-1-AP

WB analysis of SH-SY5Y using 16768-1-AP

WB analysis of SH-SY5Y using 16768-1-AP

IP experiment of HeLa using 16768-1-AP

IHC staining of human ovary tumor using 16768-1-AP

IHC staining of mouse placenta using 16768-1-AP

IF Staining of SH-SY5Y using 16768-1-AP

IF Staining of SH-SY5Y using 16768-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Biallelic COLGALT1 variants are associated with cerebral small vessel disease.

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.

Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.