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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using GLT25D2 Polyclonal antibody (25993-1-AP)

    WB analysis of SH-SY5Y using 25993-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25993-1-AP (GLT25D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using GLT25D2 Polyclonal antibody (25993-1-AP)

    WB analysis of Neuro-2a using 25993-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25993-1-AP (GLT25D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GLT25D2 Polyclonal antibody (25993-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GLT25D2 Polyclonal antibody (25993-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GLT25D2 Polyclonal antibody

GLT25D2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25993-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 2, COLGALT2, ColGalT 2, C1orf17

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, Neuro-2a cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25993-1-AP targets GLT25D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLT25D2 fusion protein Ag23360 相同性解析による交差性が予測される生物種
Full Name glycosyltransferase 25 domain containing 2
Observed molecular weight 65 kDa
GenBank accession numberBC035672
Gene Symbol GLT25D2
Gene ID (NCBI) 23127
RRIDAB_2880324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GLT25D2 antibody 25993-1-APDownload protocol
IHC protocol for GLT25D2 antibody 25993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Dis Model Mech

Loss of function of Colgalt1 disrupts collagen post-translational modification and causes musculoskeletal defects.

Authors - Krista A Geister
View Article