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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00521-3

    Sandwich ELISA standard curve of MP00521-3

    Sandwich ELISA standard curve of MP00521-3, Human GM-CSF Recombinant Matched Antibody Pair - PBS only. 82761-11-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0189. 82761-8-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Sample test of MP00521-3

    Sample test of MP00521-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean GM-CSF concentration was determined to be 55.0 pg/mL in unstimulated PBMC supernatant, 1821.6 pg/mL in PHA stimulated PBMC supernatant.

  • Cytometric bead array standard curve of MP00521-1

    Cytometric bead array standard curve of MP00521-1

    Cytometric bead array standard curve of MP00521-1, GM-CSF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82761-9-PBS. Detection antibody: 82761-8-PBS. Standard: Eg0189. Range: 0.156-20 ng/mL.

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GM-CSF Recombinant antibody, PBS Only (Detector)

GM-CSF Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 82761-8-PBS
Clone No.240183A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82761-8-PBS targets GM-CSF as part of a matched antibody pair:

MP00521-1: 82761-9-PBS capture and 82761-8-PBS detection (validated in Cytometric bead array)

MP00521-3: 82761-11-PBS capture and 82761-8-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04141
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.