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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GM130;GOLGA2 Recombinant antibody, PBS Only

GM130;GOLGA2 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1M17

Cat no : 82705-8-PBS

Synonyms

GM130, GM130 autoantigen, GOLGA2, Golgin 95, Golgin subfamily A member 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GM130;GOLGA2 Recombinant antibody (82705-8-RR)

    WB analysis using 82705-8-RR (same clone as 82705-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GM130;GOLGA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GM130;GOLGA2 Recombinant antibody (82705-8-RR)

    IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GM130;GOLGA2 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82705-8-PBS targets GM130;GOLGA2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GM130;GOLGA2 fusion protein Ag1848 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC014188
Gene symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)