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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    WB analysis using 82705-8-RR (same clone as 82705-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    WB analysis of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    WB result of GOLGA2/GM130 antibody (82705-8-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    IHC staining of rat kidney using 82705-8-RR (same clone as 82705-8-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    IHC staining of rat kidney using 82705-8-RR (same clone as 82705-8-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Recombinant antibody (82705-8-RR)

    IF Staining of HeLa using 82705-8-RR (same clone as 82705-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 82705-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82705-8-RR

    Affinity and Kinetic Characterization of 82705-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82705-8-RR against Human GM130/GOLGA2 were performed. The affinity constant is 0.942 nM.

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  • Featured Product
  • KD/KO Validated

GOLGA2/GM130 Recombinant antibody, PBS Only

GOLGA2/GM130 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82705-8-PBS
Clone No.1M17

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 1M17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82705-8-PBS targets GOLGA2/GM130 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC014188
Gene Symbol GM130
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08379
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)