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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMEB1-Specific Recombinant antibody

GMEB1-Specific Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241447C6

Cat no : 84448-1-RR

Synonyms

GMEB1, GMEB-1, GMEB 1, Glucocorticoid modulatory element-binding protein 1, DNA-binding protein p96PIF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMEB1-Specific Recombinant antibody (84448-1-RR)

    WB analysis using 84448-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using GMEB1-Specific Recombinant antibody (84448-1-RR)

    FC experiment of HeLa using 84448-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Daudi cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84448-1-RR targets GMEB1-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glucocorticoid modulatory element binding protein 1
Calculated molecular weight 63 kDa
Observed molecular weight88 kDa
GenBank accession numberNM_006582
Gene symbol GMEB1
Gene ID (NCBI) 10691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.

Protocols

Product Specific Protocols
WB protocol for GMEB1-Specific antibody 84448-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols