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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMEB2 Polyclonal antibody

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13314-1-AP

Synonyms

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of human placenta using 13314-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of mouse thymus using 13314-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of mouse ovary using 13314-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GMEB2 Polyclonal antibody (13314-1-AP)

    IF Staining of MCF-7 using 13314-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse thymus tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13314-1-AP targets GMEB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMEB2 fusion protein Ag4157 相同性解析による交差性が予測される生物種
Full Name glucocorticoid modulatory element binding protein 2
Calculated molecular weight 530 aa, 56 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC036305
Gene symbol GMEB2
Gene ID (NCBI) 26205
RRIDAB_2110829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for GMEB2 antibody 13314-1-APDownload protocol
IHC protocol for GMEB2 antibody 13314-1-APDownload protocol
IF protocol for GMEB2 antibody 13314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols