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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMF Beta Monoclonal antibody, PBS Only

GMF Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10A2

Cat no : 60062-1-PBS

Synonyms

glia maturation factor beta, GMF Beta, GMFB, GMF-beta

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using GMF Beta Monoclonal antibody (60062-1-Ig)

    WB analysis of fetal human brain using 60062-1-Ig (same clone as 60062-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GMF Beta Monoclonal antibody (60062-1-Ig)

    WB analysis of pig liver using 60062-1-Ig (same clone as 60062-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 60062-1-Ig (GMF-beta antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GMF Beta Monoclonal antibody (60062-1-Ig)

    IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GMF Beta Monoclonal antibody (60062-1-Ig)

    IHC staining of human brain using 60062-1-Ig (same clone as 60062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60062-1-Ig (GMF-beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using GMF Beta Monoclonal antibody (60062-1-Ig)

    IF Staining of U-87 MG using 60062-1-Ig (same clone as 60062-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using GMF Beta antibody (60062-1-Ig, Clone: 3B10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GMF Beta Monoclonal antibody (60062-1-Ig)

    IF Staining of HeLa using 60062-1-Ig (same clone as 60062-1-PBS)

    Immunofluorescent analysis of Hela cells, using GMFB antibody 60062-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 60062-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60062-1-PBS targets GMF Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMF Beta fusion protein Ag1104 相同性解析による交差性が予測される生物種
Full Name glia maturation factor, beta
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC005359
Gene symbol GMFB
Gene ID (NCBI) 2764
RRIDAB_2110835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.