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KD/KO Validated

GMFG Polyclonal antibody

Name: GMFG Polyclonal antibody
Brand: Proteintech
SKU: 13625-1-AP

GMFG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13625-1-AP

Synonyms

Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of rat brain using 13625-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 13625-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13625-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 13625-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 13625-1-AP
IP result of anti-GMFG (IP:13625-1-AP, 4ug; Detection:13625-1-AP 1:500) with mouse spleen tissue lysate 4000ug.
IHC staining of human lung cancer using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 13625-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 13625-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat brain tissue, human heart tissue, human placenta tissue, mouse placenta tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human lung cancer tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

13625-1-AP targets GMFG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GMFG fusion protein Ag4538 相同性解析による交差性が予測される生物種
Full Name	glia maturation factor, gamma
Calculated molecular weight	141 aa, 17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC032819
Gene symbol	GMFG
Gene ID (NCBI)	9535
RRID	AB_2110929
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GMFG, is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.

Protocols

Product Specific Protocols
WB protocol for GMFG antibody 13625-1-AP	Download protocol
IHC protocol for GMFG antibody 13625-1-AP	Download protocol
IP protocol for GMFG antibody 13625-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Development Coronary veins determine the pattern of sympathetic innervation in the developing heart. Authors - Nam Joseph J View Article
human,mouse	WB,IP	J Leukoc Biol Glia maturation factor-γ regulates amyloid-β42 phagocytosis through scavenger receptor AI in murine macrophages Authors - Wulin Aerbajinai KD Validated View Article
human	WB,IF	Front Cell Dev Biol The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse. Authors - Nikola Deretic KD Validated View Article
human	IHC	Front Oncol GMFG Has Potential to Be a Novel Prognostic Marker and Related to Immune Infiltrates in Breast Cancer. Authors - Yan Yang View Article
		Oncol Rep Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro. Authors - Huili Wang View Article
human	WB,IHC	Genes (Basel) High Level of GMFG Correlated to Poor Clinical Outcome and Promoted Cell Migration and Invasion through EMT Pathway in Triple-Negative Breast Cancer Authors - Yonglin Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMFG Polyclonal antibody

GMFG Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of rat brain using 13625-1-AP

WB analysis of human placenta using 13625-1-AP

WB analysis of human heart using 13625-1-AP

WB analysis of mouse placenta using 13625-1-AP

IP experiment of mouse spleen using 13625-1-AP

IHC staining of human lung cancer using 13625-1-AP

IHC staining of human lung cancer using 13625-1-AP

IHC staining of human heart using 13625-1-AP

IHC staining of human heart using 13625-1-AP

IHC staining of human kidney using 13625-1-AP

IHC staining of human kidney using 13625-1-AP

IHC staining of human placenta using 13625-1-AP

IHC staining of human placenta using 13625-1-AP

IHC staining of human testis using 13625-1-AP

IHC staining of human testis using 13625-1-AP

IHC staining of human spleen using 13625-1-AP

IHC staining of human spleen using 13625-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Coronary veins determine the pattern of sympathetic innervation in the developing heart.

Glia maturation factor-γ regulates amyloid-β42 phagocytosis through scavenger receptor AI in murine macrophages

The Actin-Disassembly Protein Glia Maturation Factor γ Enhances Actin Remodeling and B Cell Antigen Receptor Signaling at the Immune Synapse.

GMFG Has Potential to Be a Novel Prognostic Marker and Related to Immune Infiltrates in Breast Cancer.

Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.

High Level of GMFG Correlated to Poor Clinical Outcome and Promoted Cell Migration and Invasion through EMT Pathway in Triple-Negative Breast Cancer